Project Insight was a clandestine and secretive operation by S.H.I.E.L.D. that could eliminate any human on Earth. The operation was initiated following the Battle of New York, but its noble intentions were twisted by Hydra, who equipped Project Insight with Zola's algorithm, an algorithm capable of determining a person's threat to Hydra.
Upon discovering that Hydra had invaded S.H.I.E.L.D. and intended to deploy Project Insight, Steve Rogers, Sam Wilson, Natasha Romanoff, and Maria Hill worked together to put an end to Project Insight.